Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation

M Buisson; B Fleurent; M Mak; P Morand; L Chan; A Ng; M Guan; D Chin; J M Seigneurin

doi:10.1128/JCM.37.8.2709-2714.1999

Novel immunoblot assay using four recombinant antigens for diagnosis of Epstein-Barr virus primary infection and reactivation

J Clin Microbiol. 1999 Aug;37(8):2709-14. doi: 10.1128/JCM.37.8.2709-2714.1999.

Authors

M Buisson¹, B Fleurent, M Mak, P Morand, L Chan, A Ng, M Guan, D Chin, J M Seigneurin

Affiliation

¹ Laboratoire de Virologie Médicale Moléculaire, RHAP-CNRS, Faculté de Médecine, 38043 Grenoble, France. [email protected]

Abstract

A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with an immunofluorescence assay on a total of 291 sera. The test was accurate in 94.5% of cases of primary EBV infection, while an immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.

Publication types

Comparative Study

MeSH terms

Antigens, Viral / analysis
Antigens, Viral / immunology*
Herpesviridae Infections / diagnosis*
Herpesviridae Infections / virology*
Herpesvirus 4, Human / immunology
Herpesvirus 4, Human / isolation & purification*
Humans
Immunoblotting / methods*
Recombinant Proteins / analysis
Recombinant Proteins / immunology
Sensitivity and Specificity
Serologic Tests / methods
Tumor Virus Infections / diagnosis*
Tumor Virus Infections / virology*

Substances

Antigens, Viral
Recombinant Proteins