Abstract
Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Artemisia*
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Cell Line
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Cell Survival / drug effects
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Interferon-gamma / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages
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Mice
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Nitric Oxide Synthase / antagonists & inhibitors*
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Nitric Oxide Synthase / genetics*
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Nitric Oxide Synthase Type II
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plants, Medicinal*
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Recombinant Proteins
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Scopoletin / isolation & purification
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Scopoletin / pharmacology*
Substances
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Lipopolysaccharides
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Plant Extracts
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Recombinant Proteins
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Interferon-gamma
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Scopoletin