Combinatorial strategies for the discovery of novel protease specificities

Comb Chem High Throughput Screen. 1999 Apr;2(2):73-90.

Abstract

This article discusses proven and possible ways to generate novel cleavage specificities in serine proteases using combinatorial mutagenesis, compares the different ways of screening or selecting for desirable mutants, and examines the ways in which combinatorial substrate libraries can be used to gain a more comprehensive insight into protease cleavage preferences. The use of bacteriophage to display both combinatorial protease libraries and combinatorial substrate libraries will be discussed.

Publication types

  • Review

MeSH terms

  • Evaluation Studies as Topic
  • Mutagenesis, Site-Directed
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism*
  • Substrate Specificity

Substances

  • Serine Endopeptidases