PPARgamma activation induces the expression of the adipocyte fatty acid binding protein gene in human monocytes

Biochem Biophys Res Commun. 1999 Aug 2;261(2):456-8. doi: 10.1006/bbrc.1999.1071.

Abstract

The peroxisome-proliferator activated receptor gamma (PPARgamma), a member of the nuclear receptor superfamily of ligand activated transcription factors, plays a key role in the anti-diabetic actions of the thiazolidinediones (TZDs). PPARgamma induces the expression of many genes involved in lipid anabolism, including the adipocyte fatty acid binding protein (aP2), and is a key regulator of adipocyte differentiation. PPARgamma is also expressed in hematopoietic cells and is up-regulated in activated monocytes/macrophages. Activation of PPARgamma may play a role in the induction of differentiation of macrophages to foam cells that are associated with atherosclerotic lesions. We report that both natural and synthetic PPARgamma agonists induce time- and dose-dependent increases in aP2 mRNA in both primary human monocytes and the monocytic cell line, THP-1. These data suggest that PPARgamma activation may play a role in monocyte differentiation and function analogous to its well-characterized role in adipocytes.

MeSH terms

  • Adipocytes / metabolism*
  • Carrier Proteins / genetics*
  • Cell Differentiation / drug effects
  • Cell Line
  • Cells, Cultured
  • Chromans / pharmacology
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids / metabolism*
  • Gene Expression / drug effects
  • Humans
  • Kinetics
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Myelin P2 Protein / genetics*
  • Neoplasm Proteins*
  • Prostaglandin D2 / analogs & derivatives
  • Prostaglandin D2 / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cytoplasmic and Nuclear / agonists
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Rosiglitazone
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thiazoles / pharmacology
  • Thiazolidinediones*
  • Transcription Factors / agonists
  • Transcription Factors / metabolism*
  • Troglitazone
  • Tumor Suppressor Proteins*

Substances

  • 15-deoxy-delta(12,14)-prostaglandin J2
  • Carrier Proteins
  • Chromans
  • FABP7 protein, human
  • Fatty Acid-Binding Protein 7
  • Fatty Acid-Binding Proteins
  • Fatty Acids
  • Myelin P2 Protein
  • Neoplasm Proteins
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Thiazoles
  • Thiazolidinediones
  • Transcription Factors
  • Tumor Suppressor Proteins
  • Rosiglitazone
  • Troglitazone
  • Tetradecanoylphorbol Acetate
  • Prostaglandin D2