Abstract
Self-renewing, totipotent embryonic stem (ES) cells may provide a virtually unlimited donor source for transplantation. A protocol that permits the in vitro generation of precursors for oligodendrocytes and astrocytes from ES cells was devised. Transplantation in a rat model of a human myelin disease shows that these ES cell-derived precursors interact with host neurons and efficiently myelinate axons in brain and spinal cord. Thus, ES cells can serve as a valuable source of cell type-specific somatic precursors for neural transplantation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Astrocytes / cytology*
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Brain / embryology
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Brain / metabolism
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Cell Differentiation
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Cell Line
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Cell Movement
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Cerebral Ventricles / embryology
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Cerebral Ventricles / surgery
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Diffuse Cerebral Sclerosis of Schilder / genetics
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Diffuse Cerebral Sclerosis of Schilder / therapy*
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Embryo, Mammalian / cytology
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Growth Substances / pharmacology
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Humans
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Male
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Mice
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Myelin Basic Protein / biosynthesis
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Myelin Proteolipid Protein / biosynthesis
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Myelin Proteolipid Protein / genetics
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Myelin Sheath / physiology*
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Oligodendroglia / cytology*
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Oligodendroglia / metabolism
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Oligodendroglia / transplantation*
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Oligodendroglia / ultrastructure
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Rats
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Spinal Cord
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Stem Cell Transplantation
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Stem Cells / cytology*
Substances
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Growth Substances
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Myelin Basic Protein
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Myelin Proteolipid Protein