Down-regulation of phospholipase D during differentiation of mouse F9 teratocarcinoma cells

FEBS Lett. 1999 Jul 9;454(3):197-200. doi: 10.1016/s0014-5793(99)00798-x.

Abstract

Phospholipase D has been recognized as playing an important role in signal transduction in many types of cells. We investigated the expression of phospholipase D during the differentiation of F9 embryonal teratocarcinoma cells. The ADP ribosylation factor-dependent phospholipase D activity, as measured by an in vitro assay, and H2O2-induced phospholipase D activity and phospholipase D protein content in whole cells were decreased during the differentiation of F9 cells induced by a combination of dibutyryl cyclic AMP and all-trans retinoic acid. In contrast, these changes were not observed when cells were induced by retinoic acid. These results suggest that down-regulation of phospholipase D protein is associated with differentiation of F9 cells to a parietal endoderm lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Cell Differentiation / drug effects
  • Cyclic AMP / pharmacology
  • Down-Regulation / drug effects
  • Mice
  • Phospholipase D / biosynthesis*
  • Teratocarcinoma / enzymology*
  • Teratocarcinoma / pathology*
  • Tretinoin / pharmacology
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents
  • Tretinoin
  • Cyclic AMP
  • Phospholipase D