Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

P de Felipe; V Martín; M L Cortés; M Ryan; M Izquierdo

doi:10.1038/sj.gt.3300811

Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Gene Ther. 1999 Feb;6(2):198-208. doi: 10.1038/sj.gt.3300811.

Authors

P de Felipe¹, V Martín, M L Cortés, M Ryan, M Izquierdo

Affiliation

¹ Departamento de Bioquímica y Biología Molecular, Universidad Autónoma de Madrid, Facultad de Ciencias, Spain.

PMID: 10435104
DOI: 10.1038/sj.gt.3300811

Abstract

We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Acetyltransferases / genetics
Animals
Antiviral Agents / pharmacology
Aphthovirus / genetics*
Blotting, Western
Chimera
Ganciclovir / pharmacology
Gene Expression
Genetic Therapy / methods*
Genetic Vectors*
Genome, Viral
Humans
Mice
Plasmids
Thymidine Kinase / genetics
Tumor Cells, Cultured

Substances

Antiviral Agents
Acetyltransferases
puromycin N-acetyltransferase
Thymidine Kinase
Ganciclovir