Abstract
The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Cysteine Endopeptidases / chemistry
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Cysteine Endopeptidases / genetics*
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Cysteine Endopeptidases / isolation & purification
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DNA Primers / genetics
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DNA, Complementary / genetics
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Mice
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Molecular Sequence Data
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Multienzyme Complexes / chemistry
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Multienzyme Complexes / genetics*
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Multienzyme Complexes / isolation & purification
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Phylogeny
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Proteasome Endopeptidase Complex
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Protein Conformation
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Rats
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Sequence Homology, Amino Acid
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Species Specificity
Substances
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DNA Primers
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DNA, Complementary
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Multienzyme Complexes
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Cysteine Endopeptidases
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Proteasome Endopeptidase Complex
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Psmb5 protein, mouse
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Psmb5 protein, rat
Associated data
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GENBANK/AF019661
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GENBANK/AF019662
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GENBANK/AF060087
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GENBANK/AF060088
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GENBANK/AF060089
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GENBANK/AF060090
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GENBANK/AF060091
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GENBANK/AF060092
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GENBANK/AF060093
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GENBANK/AF099733