Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

T Lioux; I Lefebvre; J J Vasseur; J L Imbach

doi:10.1002/(SICI)1097-0231(19990830)13:16<1645::AID-RCM692>3.0.CO;2-Y

Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

Rapid Commun Mass Spectrom. 1999;13(16):1645-9. doi: 10.1002/(SICI)1097-0231(19990830)13:16<1645::AID-RCM692>3.0.CO;2-Y.

Authors

T Lioux¹, I Lefebvre, J J Vasseur, J L Imbach

Affiliation

¹ Laboratoire de Chimie Bioorganique, U.M.R. C.N.R.S. 5625, Case Courrier 008, Université Montpellier II, Place Eugéne Bataillon, 34095 Montpellier Cedex 5, France.

Abstract

Using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) we studied the decomposition pathway of a prooligonucleotide during incubation in a crude cell extract. This study, involving no sample processing, except drop dailysis to remove salts, led us to identify more than thirty metabolites from several metabolic pathways.

MeSH terms

Animals
Cell Line
Oligonucleotides / chemistry
Oligonucleotides / metabolism*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

Oligonucleotides