Experimental cirrhosis of the liver and cytoprotective effects of alpha tocopherol

East Afr Med J. 1999 Apr;76(4):223-7.

Abstract

Objective: To investigate whether alpha tocopherol (alpha-T) treatment influences liver cirrhosis induced by carbon tetrachloride (CCl4) in the rat.

Design: Laboratory experiment.

Setting: Teaching hospital, Turkey.

Subjects: Fifty rats were divided into three groups. Group I consisted of ten controls; Group II: twenty CCl4 induced cirrhotic rats and Group III: twenty cirrhotic rats treated with Vitamin E.

Interventions: Experimental cirrhosis was induced in rats by subcutaneous administration of 0.4 ml CCl4 (diluted 1:4 with olive oil) per kilogram body weight twice a week for 12 weeks.

Main outcome measures: Histopathologically evaluation of liver, liver malondialdehyde and glutathione peroxidase levels and aspartate aminotransferase (SGOT), alanine aminotransferase (SGPT) and gamma-glutamyltranspeptidase (GGT) levels.

Results: Histopathologic evaluation of liver samples demonstrated cirrhotic changes in CCl4 treated groups. The enzyme levels in Group II were significantly increased (p < 0.05) but it was insignificantly lower in the alpha tocopherol treated group III. In Group II, statistically significant increase in liver malondialdehyde levels was determined (p < 0.001).

Conclusion: Our results demonstrate that alpha-T administration may protect liver against CCl4 induced cirrhosis.

MeSH terms

  • Analysis of Variance
  • Animals
  • Antioxidants / therapeutic use*
  • Carbon Tetrachloride
  • Liver / drug effects*
  • Liver Cirrhosis, Experimental / chemically induced
  • Liver Cirrhosis, Experimental / prevention & control*
  • Male
  • Rats
  • Rats, Wistar
  • Statistics, Nonparametric
  • Vitamin E / therapeutic use*

Substances

  • Antioxidants
  • Vitamin E
  • Carbon Tetrachloride