Enhanced GRK5 expression in the hearts of cardiomyopathic hamsters, J2N-k

Biochem Biophys Res Commun. 1999 Aug 19;262(1):206-10. doi: 10.1006/bbrc.1999.1179.

Abstract

GTP binding protein-coupled receptor kinase 5 (GRK5) cDNA was cloned from the hearts of Syrian hamsters. The hamster GRK5 cDNA contained 1770 nucleotides encoding 590 amino acids, and the nucleotide sequence had 89.6% homology to the human homologue. An inbred cardiomyopathic hamster strain, J2N-k, was used to investigate the alteration of GRK5 mRNA expression in the setting of congestive heart failure. M-mode echocardiography revealed significant dilatation of the left ventricle and a decrease of left ventricular contractility in 20-week-old J2N-k hamsters compared with age-matched control hamsters, J2N-n. Semi-quantitative RT-PCR showed that GRK5 mRNA expression in the hearts of J2N-k was significantly higher than in those of J2N-n (J2N-k 60.3 +/- 13.3, J2N-n 25.8 +/- 17.2 arbitrary units, p < 0.005, n = 6 in each group). These results suggest that an enhanced GRK5 expression might play a role in the reduced responsiveness to catecholamines in failing hearts via beta-adrenergic receptor phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cricetinae
  • Disease Models, Animal
  • Female
  • G-Protein-Coupled Receptor Kinase 5
  • Gene Expression Regulation, Enzymologic
  • Heart Failure / enzymology
  • Heart Failure / genetics*
  • Heart Failure / pathology
  • Heart Failure / physiopathology
  • Heart Ventricles / diagnostic imaging
  • Heart Ventricles / enzymology
  • Heart Ventricles / pathology
  • Heart Ventricles / physiopathology
  • Humans
  • Male
  • Mesocricetus / genetics*
  • Molecular Sequence Data
  • Myocardial Contraction
  • Myocardium / enzymology*
  • Myocardium / pathology
  • Organ Size
  • Protein Serine-Threonine Kinases*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptor Protein-Tyrosine Kinases / chemistry
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Sequence Homology, Amino Acid
  • Ultrasonography
  • Up-Regulation*

Substances

  • RNA, Messenger
  • Receptor Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • G-Protein-Coupled Receptor Kinase 5
  • GRK5 protein, human