Oligonucleotide-peptide conjugates as potential antisense agents

E M Zubin; E A Romanova; E M Volkov; V N Tashlitsky; G A Korshunova; Z A Shabarova; T S Oretskaya

doi:10.1016/s0014-5793(99)00921-7

Oligonucleotide-peptide conjugates as potential antisense agents

FEBS Lett. 1999 Jul 30;456(1):59-62. doi: 10.1016/s0014-5793(99)00921-7.

Authors

E M Zubin¹, E A Romanova, E M Volkov, V N Tashlitsky, G A Korshunova, Z A Shabarova, T S Oretskaya

Affiliation

¹ Chemical Department and A.N. Belozersky Institute of Physical Chemical Biology, M.V. Lomonosov Moscow State University, Russia.

PMID: 10452530
DOI: 10.1016/s0014-5793(99)00921-7

Abstract

Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized peptides to the 2'-position of a selected nucleotide within an otherwise protected oligonucleotide chain attached to a solid support.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine / analogs & derivatives
Adenosine / chemistry
Arabinonucleosides / chemistry
Biochemistry / methods*
Chromatography, High Pressure Liquid
Nucleoproteins / chemical synthesis
Nucleoproteins / chemistry*
Oligonucleotides / chemical synthesis
Oligonucleotides / chemistry*
Oligonucleotides, Antisense / chemical synthesis
Oligonucleotides, Antisense / chemistry*
Peptides / chemical synthesis
Peptides / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

2'-amino-2'-deoxyarabinoadenosine
Arabinonucleosides
Nucleoproteins
Oligonucleotides
Oligonucleotides, Antisense
Peptides
Adenosine