Abstract
The beta3-adrenoceptor (AR) differs from the beta1-AR and beta2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse beta3-AR which differ within the coding region. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse beta3-adrenoceptor. The novel beta3b-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known beta3a-AR (RFDGYEGARPFPT). Beta3b-AR mRNA is differentially expressed in mouse tissues, with levels relative to beta3a-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / metabolism
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Cerebral Cortex / metabolism
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation
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Hypothalamus / metabolism
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Ileum / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Muscle, Smooth / metabolism
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Protein Isoforms / genetics
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Adrenergic, beta / genetics*
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Receptors, Adrenergic, beta-3
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Tissue Distribution
Substances
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DNA, Complementary
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Protein Isoforms
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RNA, Messenger
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Receptors, Adrenergic, beta
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Receptors, Adrenergic, beta-3