Identification of a novel gene of the X,K-ATPase beta-subunit family that is predominantly expressed in skeletal and heart muscles

FEBS Lett. 1999 Aug 6;456(2):243-8. doi: 10.1016/s0014-5793(99)00954-0.

Abstract

We have identified the fifth member of the mammalian X,K-ATPase beta-subunit gene family. The human and rat genes are largely expressed in skeletal muscle and at a lower level in heart. The deduced human and rat proteins designated as beta(muscle) (beta(m)) consist of 357 and 356 amino acid residues, respectively, and exhibit 89% identity. The sequence homology of beta(m) proteins with known Na,K- and H,K-ATPase beta-subunits are 30.5-39.4%. Unlike other beta-subunits, putative beta(m) proteins have large N-terminal cytoplasmic domains containing long Glu-rich sequences. The data obtained indicate the existence of hitherto unknown X,K-ATPase (most probably Na,K-ATPase) isozymes in muscle cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Conserved Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Gene Expression
  • H(+)-K(+)-Exchanging ATPase / chemistry
  • H(+)-K(+)-Exchanging ATPase / genetics*
  • Humans
  • Isoenzymes / chemistry
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Multigene Family*
  • Muscle, Skeletal / enzymology*
  • Myocardium / enzymology*
  • Protein Conformation
  • Rats
  • Sequence Homology, Amino Acid
  • Sodium-Potassium-Exchanging ATPase / chemistry
  • Sodium-Potassium-Exchanging ATPase / genetics*
  • Species Specificity
  • Tissue Distribution

Substances

  • DNA Primers
  • DNA, Complementary
  • Isoenzymes
  • H(+)-K(+)-Exchanging ATPase
  • Sodium-Potassium-Exchanging ATPase

Associated data

  • GENBANK/AF015383
  • GENBANK/AF015384
  • GENBANK/AF015385
  • GENBANK/AF015386