Abstract
We have identified the fifth member of the mammalian X,K-ATPase beta-subunit gene family. The human and rat genes are largely expressed in skeletal muscle and at a lower level in heart. The deduced human and rat proteins designated as beta(muscle) (beta(m)) consist of 357 and 356 amino acid residues, respectively, and exhibit 89% identity. The sequence homology of beta(m) proteins with known Na,K- and H,K-ATPase beta-subunits are 30.5-39.4%. Unlike other beta-subunits, putative beta(m) proteins have large N-terminal cytoplasmic domains containing long Glu-rich sequences. The data obtained indicate the existence of hitherto unknown X,K-ATPase (most probably Na,K-ATPase) isozymes in muscle cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Conserved Sequence
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DNA Primers / genetics
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DNA, Complementary / genetics
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Gene Expression
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H(+)-K(+)-Exchanging ATPase / chemistry
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H(+)-K(+)-Exchanging ATPase / genetics*
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Humans
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Isoenzymes / chemistry
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Isoenzymes / genetics*
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Molecular Sequence Data
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Multigene Family*
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Muscle, Skeletal / enzymology*
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Myocardium / enzymology*
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Protein Conformation
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Rats
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Sequence Homology, Amino Acid
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Sodium-Potassium-Exchanging ATPase / chemistry
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Sodium-Potassium-Exchanging ATPase / genetics*
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Species Specificity
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Tissue Distribution
Substances
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DNA Primers
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DNA, Complementary
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Isoenzymes
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H(+)-K(+)-Exchanging ATPase
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Sodium-Potassium-Exchanging ATPase
Associated data
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GENBANK/AF015383
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GENBANK/AF015384
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GENBANK/AF015385
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GENBANK/AF015386