Use of reverse transcription polymerase chain reaction with colorimetric plate hybridization to detect a cytomegalovirus late spliced mRNA in polymorphonuclear leukocytes from renal transplant patients

E Andre; B M Imbert-Marcille; D Cantarovich; B Besse; V Ferre-Aubineau; S Billaudel

doi:10.1016/s0732-8893(99)00043-7

Use of reverse transcription polymerase chain reaction with colorimetric plate hybridization to detect a cytomegalovirus late spliced mRNA in polymorphonuclear leukocytes from renal transplant patients

Diagn Microbiol Infect Dis. 1999 Aug;34(4):287-91. doi: 10.1016/s0732-8893(99)00043-7.

Authors

E Andre¹, B M Imbert-Marcille, D Cantarovich, B Besse, V Ferre-Aubineau, S Billaudel

Affiliation

¹ Virology Laboratory, Nantes University Hospital, France.

PMID: 10459479
DOI: 10.1016/s0732-8893(99)00043-7

Abstract

Human cytomegalovirus replication was evaluated in polymorphonuclear leukocytes from ten renal transplant recipients. Three new reverse transcription polymerase chain reactions with plate hybridization suitable for automation were developed for the detection of immediate-early spliced UL123 mRNA, early-late pp65 mRNA, and late spliced UL22 mRNA. The presence of UL22mRNA was found to be significantly associated with the occurrence of cytomegalovirus (CMV) disease.

MeSH terms

Colorimetry / methods*
Cytomegalovirus Infections / complications
Cytomegalovirus Infections / diagnosis*
Humans
Kidney Transplantation*
Neutrophils / virology*
Nucleic Acid Hybridization / methods*
Opportunistic Infections / complications
Opportunistic Infections / diagnosis*
Reverse Transcriptase Polymerase Chain Reaction* / methods
Reverse Transcriptase Polymerase Chain Reaction* / standards
Transcription, Genetic