Detection of glycopeptide-resistant enterococci in routine diagnostic faeces specimens

J Hosp Infect. 1999 Sep;43(1):25-32. doi: 10.1053/jhin.1999.0630.

Abstract

Faeces received in a diagnostic laboratory were screened for glycopeptide-resistant enterococci (GRE) on modified Lewisham medium, with and without enrichment in Enterococcosel broth. Colonization by GRE was detected in 102/838 patients (12.2%). In 74 (73%) of colonized patients GRE were detected by both methods and in 28 (27%) they were detected only after enrichment. The carriage rate in hospitalized patients was 32% (93/289) compared with 2.3% (11/425) in the community (GP patients and food-handlers). Carriage of GRE increased with age. Clostridium difficile isolation was associated with GRE colonization, odds ratio 6.76 (P<0.001). Fifty-nine percent (60/102) of the GRE had the VanA phenotype and 41% (42/102) had the VanB phenotype. In the community VanA predominated (91%), whereas 64% (57/89) of the isolates from hospitalised patients were of the VanB phenotype.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology*
  • Carrier State / epidemiology*
  • Child
  • Child, Preschool
  • Clostridioides difficile / isolation & purification
  • Diagnostic Tests, Routine
  • Enterococcus / classification
  • Enterococcus / drug effects*
  • Enterococcus / isolation & purification*
  • Feces / microbiology*
  • Female
  • Genotype
  • Glycopeptides*
  • Gram-Positive Bacterial Infections / epidemiology*
  • Humans
  • Infant
  • London / epidemiology
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Phenotype

Substances

  • Anti-Bacterial Agents
  • Glycopeptides