Abstract
The fibronectin promoter contains an ATF/cyclic AMP (cAMP) response element (CRE) site two helical turns upstream of a CCAAT site with which it interacts. We investigated the effects of mutating these (-170) CRE and(-150) CCAAT elements on the promoter activity regulated by three different modulators previously known to act through CRE: ATF-2, cAMP and E1a. While the cooperation seems to play no role in E1a action, integrity of the (-150) CCAAT is necessary for ATF-2 and cAMP efficient activation in a cell-specific manner. These results show that the CRE and CCAAT elements function as a 'composite element' and establish a cell-specific function for CRE-CCAAT synergy.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells / metabolism
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Activating Transcription Factor 2
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Adenovirus E1A Proteins / genetics
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Adenovirus E1A Proteins / metabolism
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Animals
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Carcinoma, Hepatocellular / genetics
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Carcinoma, Hepatocellular / metabolism
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Cyclic AMP / metabolism*
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Cyclic AMP Response Element-Binding Protein / genetics
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Cyclic AMP Response Element-Binding Protein / metabolism*
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Cyclic AMP-Dependent Protein Kinases / metabolism
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Fibronectins / genetics*
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Fibronectins / metabolism
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Gene Expression Regulation
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Humans
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Mice
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Mutation
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Promoter Regions, Genetic
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RNA, Antisense / genetics
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Response Elements / genetics*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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ATF2 protein, human
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Activating Transcription Factor 2
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Adenovirus E1A Proteins
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Atf2 protein, mouse
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Cyclic AMP Response Element-Binding Protein
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Fibronectins
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RNA, Antisense
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Recombinant Proteins
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Transcription Factors
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Cyclic AMP
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Cyclic AMP-Dependent Protein Kinases