Frequency of p53 codon 72 genotypes in human papillomavirus associated squamous intraepithelial lesions and cervical cancer

Anticancer Res. 1999 May-Jun;19(3B):2377-9.

Abstract

Background: A recent study suggested that the p53Arg (at residue 72) homozygous genotype could be a potential genetic risk factor for cervical cancer among white women. To confirm this result we examined the proportion of p53 genotypes in a larger number of patients with cervical cancer and in patients with squamous intraepithelial lesions (SIL) compared to a control population.

Materials and methods: We used allele-specific primers to amplify the p53Arg and p53Pro sequences and we examined the proportion of genotypes in the study populations using chi 2-test.

Results: The distributions of p53Arg homozygous, heterozygous and p53Pro homozygous genotypes were 63%, 27% and 10% in cervical cancer patients, 53%, 36% and 8% in individuals with SIL, and 60%, 36% and 4% in control population. Using chi-square test, no significant difference was found between genotype frequencies in the study groups.

Conclusion: Thus, the p53Arg homozygous genotype does not seem to increase the risk of cervical cancer in Hungarian women.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arginine
  • Carcinoma, Squamous Cell / epidemiology
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / virology*
  • Chi-Square Distribution
  • Codon
  • DNA Primers
  • Female
  • Gene Frequency
  • Genes, p53*
  • Genotype
  • Heterozygote
  • Homozygote
  • Humans
  • Hungary / epidemiology
  • Papillomaviridae*
  • Polymerase Chain Reaction
  • Risk Factors
  • Uterine Cervical Neoplasms / epidemiology
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / virology*

Substances

  • Codon
  • DNA Primers
  • Arginine