High-density nucleoside analog probe arrays for enhanced hybridization

Nucleosides Nucleotides. 1999 Jun-Jul;18(6-7):1293-5. doi: 10.1080/07328319908044695.

Abstract

DNA probe arrays were synthesized with analogs of 2,6-diaminopurine and 2'-O-methyl-thymidine in place of A and T. AT-rich GeneChip test arrays containing 14-mer or 20-mer analog probes improved hybridization to fluorescently-labeled RNA sequences under stringent conditions.

MeSH terms

  • Indicators and Reagents
  • Nucleic Acid Hybridization*
  • Oligonucleotide Probes*

Substances

  • Indicators and Reagents
  • Oligonucleotide Probes