Abstract
After a brief exposure to supra-saturating light, leaves of a tobacco transformant, in which chloroplastic NAD(P)H dehydrogenase (NDH) was defective, showed more severe photoinhibition than the wild-type, when judged by the parameter of chlorophyll fluorescence Fv/Fm. Repeated application of supra-saturating light eventually resulted in chlorosis in the NDH-defective mutant, while the wild-type sustained less photodamage and was able to recover from it. The mechanism of the phenomena is discussed with respect to the potential role of NDH in photosynthesis.
MeSH terms
-
Chloroplasts / enzymology*
-
Ferredoxin-NADP Reductase / metabolism
-
Light / adverse effects*
-
Light-Harvesting Protein Complexes
-
Mutagenesis, Insertional
-
NADPH Dehydrogenase / physiology*
-
Nicotiana / enzymology
-
Nicotiana / radiation effects
-
Oxidation-Reduction
-
Photosynthesis / physiology
-
Photosynthetic Reaction Center Complex Proteins / metabolism
-
Plant Proteins / physiology
-
Plants, Toxic
-
Time Factors
-
Transformation, Genetic
Substances
-
Light-Harvesting Protein Complexes
-
Photosynthetic Reaction Center Complex Proteins
-
Plant Proteins
-
Ferredoxin-NADP Reductase
-
NADPH Dehydrogenase