Abstract
We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two residues, to that of the two class III POU proteins Cf1-a (Drosophila) and POU-M1 (Bombyx mori). Southern blot analysis suggests that crustaceans have only one class III POU gene. RT-PCR and whole-mount in situ hybridization show that APH-1 mRNA is present in larvae specifically in the salt gland, an organ which is involved in osmoregulation, and disappears in the adult.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Artemia / genetics*
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Artemia / metabolism
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Base Sequence
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Blotting, Southern
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Caenorhabditis elegans Proteins*
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DNA Restriction Enzymes / metabolism
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Gene Expression*
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / metabolism*
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In Situ Hybridization
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Molecular Sequence Data
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Reverse Transcriptase Polymerase Chain Reaction
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Salt Gland / metabolism*
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Time Factors
Substances
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APH-1 protein, C elegans
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Caenorhabditis elegans Proteins
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Homeodomain Proteins
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DNA Restriction Enzymes