Membrane filter assay for detection of amyloid-like polyglutamine-containing protein aggregates

Methods Enzymol. 1999:309:375-86. doi: 10.1016/s0076-6879(99)09026-6.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amyloid / chemistry
  • Amyloid / isolation & purification*
  • Escherichia coli
  • Filtration*
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / genetics
  • Humans
  • Huntingtin Protein
  • Immunoblotting / methods
  • Membranes, Artificial
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification*
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification*
  • Peptides / chemistry
  • Peptides / isolation & purification*
  • Protein Conformation
  • Recombinant Fusion Proteins / chemistry

Substances

  • Amyloid
  • HTT protein, human
  • Huntingtin Protein
  • Membranes, Artificial
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Peptides
  • Recombinant Fusion Proteins
  • polyglutamine
  • Glutathione Transferase