Abstract
The Friend virus susceptibility 2 (Fv2) locus encodes a dominant host factor that confers susceptibility to Friend virus-induced erythroleukaemia in mice. We mapped Fv2 to a 1.0-Mb interval that also contained the gene (Ron) encoding the stem cell kinase receptor (Stk). A truncated form of Stk (Sf-stk), which was the most abundant form of Stk in Fv2-sensitive (Fv2ss) erythroid cells, was not expressed in Fv2 resistant (Fv2rr) cells. Enforced expression of Sf-stk conferred susceptibility to Friend disease, whereas targeted disruption of Ron caused resistance. We conclude that the Fv2 locus encodes Ron, and that a naturally expressed, truncated form of Stk confers susceptibility to Friend virus-induced erythroleukaemia.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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Contig Mapping
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Friend murine leukemia virus*
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Gene Expression
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Genetic Predisposition to Disease
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Leukemia, Erythroblastic, Acute / genetics*
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Mice
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Mice, Inbred AKR
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred CBA
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Mice, Inbred NZB
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Mice, Inbred Strains
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Molecular Sequence Data
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Muridae
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Protein Isoforms / genetics
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptors, Cell Surface / genetics*
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Retroviridae Infections / genetics
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Species Specificity
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Spleen / cytology
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Spleen / metabolism
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Spleen / pathology
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Tumor Virus Infections / genetics
Substances
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Protein Isoforms
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Receptors, Cell Surface
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RON protein
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Receptor Protein-Tyrosine Kinases