Oligonucleotides containing 4'-C-aminomethyl-2'-modified thymidines show increased binding affinity towards DNA and RNA

H M Pfundheller; J Wengel

doi:10.1016/s0960-894x(99)00452-7

Oligonucleotides containing 4'-C-aminomethyl-2'-modified thymidines show increased binding affinity towards DNA and RNA

Bioorg Med Chem Lett. 1999 Sep 20;9(18):2667-72. doi: 10.1016/s0960-894x(99)00452-7.

Authors

H M Pfundheller¹, J Wengel

Affiliation

¹ Department of Chemistry, University of Southern Denmark, Odense University.

PMID: 10509913
DOI: 10.1016/s0960-894x(99)00452-7

Abstract

Oligonucleotides containing 4'-C-aminomethyl-2'-O-methyl or 4'-C-aminomethyl-2'-deoxy-2'-fluoro modified thymidines have been synthesized. Compared with the corresponding oligodeoxynucleotide reference these novel oligonucleotide analogues display increased binding affinity towards complementary single stranded DNA as well as RNA. The possible effect of the positively charged 4'-C-aminomethyl group has been investigated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA / metabolism*
Nucleic Acid Conformation
Oligonucleotides / chemistry*
Oligonucleotides / metabolism
RNA / metabolism*
Thymidine / analysis*

Substances

Oligonucleotides
RNA
DNA
Thymidine