Abstract
Human herpesvirus-8 (HHV-8) genome encodes for genes homologous to human cellular genes such as interleukin-6 (IL-6), Cyclin-D, BCL-2, and IL-8 receptor (G-protein-coupled receptor [GCR]). We used reverse transcriptase-polymerase chain reaction to study the expression of these viral genes in lymphoproliferative disorders associated with HHV-8 infection. None of these genes was expressed in 1 case of benign, localized Castleman's disease (CD), and only viral IL-6 and viral Cyclin-D were transcribed in 2 cases of benign lymphadenopathies with giant germinal center hyperplasia and increased vascularity. In contrast, all 4 genes were transcribed in 1 case of multicentric CD of plasma cell type with aggressive clinical course and in 1 primary effusion lymphoma cell line. Our study provides the evidence that various HHV-8 genes, homologous to cellular genes involved in control of proliferation and apoptosis, may be differently expressed in different lymphoid disorders in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / biosynthesis
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Antigens, CD / genetics
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Apoptosis
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Castleman Disease / classification
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Castleman Disease / genetics
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Castleman Disease / metabolism
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Castleman Disease / pathology
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Castleman Disease / virology*
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Cell Division
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Cyclin D
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Cyclins / biosynthesis
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Cyclins / genetics
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Genes, bcl-2
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HIV / isolation & purification*
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Herpesvirus 8, Human / genetics*
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Herpesvirus 8, Human / isolation & purification
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Humans
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Interleukin-6 / biosynthesis
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Interleukin-6 / genetics
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Lymphatic Diseases / genetics
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Lymphatic Diseases / metabolism
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Lymphatic Diseases / pathology
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Lymphatic Diseases / virology
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Lymphoid Tissue / metabolism*
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Lymphoid Tissue / virology
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Lymphoproliferative Disorders / genetics
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Lymphoproliferative Disorders / metabolism
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Lymphoproliferative Disorders / virology*
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin / genetics
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Receptors, Interleukin-8A
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Reverse Transcriptase Polymerase Chain Reaction
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Species Specificity
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Transcription, Genetic
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Viral Proteins / biosynthesis*
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Viral Proteins / genetics
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Virus Latency
Substances
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Antigens, CD
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Cyclin D
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Cyclins
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Interleukin-6
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Receptors, Interleukin
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Receptors, Interleukin-8A
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Viral Proteins