Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe

J Clin Microbiol. 1999 Nov;37(11):3569-71. doi: 10.1128/JCM.37.11.3569-3571.1999.

Abstract

We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV-1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Viral / genetics*
  • DNA, Viral / isolation & purification*
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Female
  • HIV Infections / diagnosis
  • HIV-1 / classification
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification*
  • Humans
  • Infant, Newborn
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Postpartum Period
  • Pregnancy
  • Sensitivity and Specificity
  • Zimbabwe

Substances

  • DNA Primers
  • DNA, Viral