Identification of synthetic by-products in combinatorial libraries using high performance liquid chromatography-electrospray ionization mass spectrometry

Comb Chem High Throughput Screen. 1999 Oct;2(5):289-96.

Abstract

High performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI) and high performance liquid chromatography coupled to mass spectrometry (LC-MS) were used to analyze randomly chosen samples from parallel syntheses carried out on derivatized polypropylene crowns compatible with a Multipin solid support system. Side-reactions and by-products were clearly identified, and the yields of the expected molecules were unexpectedly low for most samples. LC-MS was superior to HPLC with absorbance detection or electrospray mass spectrometry alone for determining the identity and purity of each desired combinatorial compounds.

MeSH terms

  • Acrylamides / chemistry
  • Amino Acids / chemistry
  • Chromatography, High Pressure Liquid / methods*
  • Combinatorial Chemistry Techniques*
  • Ethers, Cyclic / chemistry
  • Fluorenes / chemistry
  • Mass Spectrometry / methods
  • Molecular Structure
  • Peptide Library
  • Polypropylenes / chemistry

Substances

  • Acrylamides
  • Amino Acids
  • Ethers, Cyclic
  • Fluorenes
  • N(alpha)-fluorenylmethyloxycarbonylamino acids
  • Peptide Library
  • Polypropylenes