A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors

Biochim Biophys Acta. 1999 Nov 16;1435(1-2):147-52. doi: 10.1016/s0167-4838(99)00203-4.

Abstract

We have cloned a novel 100-kDa mammalian protein, which was recognized by an anti-peptide antibody against an epitope-containing nuclear localization signal of NF-kappaB p65 subunit. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies / immunology
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / isolation & purification
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • Epitopes / immunology
  • Fungal Proteins / chemistry
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • NF-kappa B / immunology
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / isolation & purification
  • Saccharomyces cerevisiae
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins*
  • Sequence Alignment
  • Transcription Factor RelA

Substances

  • Antibodies
  • Carrier Proteins
  • DNA, Complementary
  • Epitopes
  • Fungal Proteins
  • NF-kappa B
  • Nuclear Proteins
  • PRP1 protein, S pombe
  • Schizosaccharomyces pombe Proteins
  • Transcription Factor RelA

Associated data

  • GENBANK/AB019219