Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anaerobiosis
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Animals
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Base Sequence
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Ciliophora / enzymology*
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Ciliophora / genetics
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DNA, Complementary / genetics
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Electrophoresis, Polyacrylamide Gel
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Glucans / metabolism
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Gram-Positive Bacteria / enzymology
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Gram-Positive Bacteria / genetics
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Immunoblotting
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Molecular Sequence Data
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Phylogeny
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Rumen / parasitology*
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Sequence Analysis, DNA
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Xylan Endo-1,3-beta-Xylosidase
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Xylans / metabolism
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Xylosidases / chemistry
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Xylosidases / genetics*
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Xylosidases / metabolism*
Substances
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DNA, Complementary
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Glucans
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Xylans
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Xylosidases
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Xylan Endo-1,3-beta-Xylosidase