Abstract
Cystic fibrosis (CF) patients develop chronic airway infections with Pseudomonas aeruginosa (PA). Pseudomonas aeruginosa synthesized lipopolysaccharide (LPS) with a variety of penta- and hexa-acylated lipid A structures under different environmental conditions. CF patient PA synthesized LPS with specific lipid A structures indicating unique recognition of the CF airway environment. CF-specific lipid A forms containing palmitate and aminoarabinose were associated with resistance to cationic antimicrobial peptides and increased inflammatory responses, indicating that they are likely to be involved in airway disease.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acylation
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Antimicrobial Cationic Peptides
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Arabinose / analogs & derivatives
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Arabinose / analysis
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Arabinose / metabolism
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology
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Cells, Cultured
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Cystic Fibrosis / complications
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Cystic Fibrosis / microbiology*
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Drug Resistance, Microbial
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Humans
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Infant
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Interleukin-8 / biosynthesis
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Lipid A / biosynthesis*
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Lipid A / chemistry*
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Lipopolysaccharides / chemistry
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Lipopolysaccharides / immunology
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Magnesium / pharmacology
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Mutation
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Palmitates / analysis
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Palmitates / metabolism
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Peptides / pharmacology
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Polymyxins / pharmacology
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Pseudomonas Infections / microbiology*
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Pseudomonas aeruginosa / drug effects
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / metabolism*
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Pseudomonas aeruginosa / pathogenicity
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Respiratory System / microbiology*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Virulence
Substances
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Antimicrobial Cationic Peptides
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Bacterial Proteins
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C18G peptide
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Interleukin-8
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Lipid A
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Lipopolysaccharides
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Palmitates
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Peptides
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PhoQ protein, Bacteria
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Polymyxins
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aminoarabinose
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PhoP protein, Bacteria
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Arabinose
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Magnesium