Abstract
A series of phospholipids, including previously undescribed compounds 4-7, were isolated by a bioactivity-guided fractionation from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis in human liver cells. These compounds were identified as lyso-PAF analogues (1-5) and lysophosphatidylcholines (6, 7) based on NMR and MS analyses. Compounds 1-7 specifically blocked the conversion of lanosterol into cholesterol in the Chang liver cell.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anticholesteremic Agents / isolation & purification
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Anticholesteremic Agents / pharmacology*
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Cell Line
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Cells, Cultured
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Cholesterol / biosynthesis*
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Depression, Chemical
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Humans
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Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology
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Inflammation Mediators / chemistry
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Inflammation Mediators / isolation & purification
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Inflammation Mediators / pharmacology*
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Lanosterol / biosynthesis
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Liver / drug effects
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Liver / enzymology
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Liver / metabolism*
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Lovastatin / analogs & derivatives
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Lovastatin / pharmacology
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Lysophosphatidylcholines / chemistry
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Lysophosphatidylcholines / isolation & purification
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Lysophosphatidylcholines / pharmacology*
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Magnetic Resonance Spectroscopy
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Platelet Activating Factor / analogs & derivatives*
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Platelet Activating Factor / chemistry
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Platelet Activating Factor / isolation & purification
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Platelet Activating Factor / pharmacology
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Porifera / chemistry
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Porifera / metabolism*
Substances
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Anticholesteremic Agents
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Hydroxymethylglutaryl-CoA Reductase Inhibitors
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Inflammation Mediators
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Lysophosphatidylcholines
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O-deacetyl platelet activating factor
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Platelet Activating Factor
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Lanosterol
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mevastatin
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Cholesterol
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Lovastatin