A new regulatory domain on the TATA-binding protein

EMBO J. 1999 Dec 1;18(23):6662-71. doi: 10.1093/emboj/18.23.6662.

Abstract

Recognition of the TATA box by the TATA-binding protein (TBP) is a highly regulated step in RNA polymerase II-dependent transcription. Several proteins have been proposed to regulate TBP activity, yet the TBP domains responsive to all these regulators have not been defined. Here we describe a new class of TBP mutants that increase transcription from core promoters in vivo. The majority of these mutations alter amino acids that cluster tightly on the TBP surface, defining a new TBP regulatory domain. The mutant TBP proteins are defective for binding the transcriptional regulator yNC2, are resistant to inhibition by yNC2 in vitro and exhibit allele-specific genetic interactions with yNC2. These results provide strong biochemical and genetic evidence that TBP is directly repressed in vivo, and define a new TBP domain important for transcriptional regulation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases
  • Alleles
  • DNA Helicases / metabolism
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation, Fungal
  • Genetic Complementation Test
  • Models, Molecular
  • Mutagenesis
  • Phenotype
  • Phosphoproteins / chemistry
  • Phosphoproteins / metabolism
  • Plasmids
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Structure, Tertiary
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae Proteins*
  • TATA-Binding Protein Associated Factors*
  • TATA-Box Binding Protein
  • Transcription Factor TFIIA
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • BUR6 protein, S cerevisiae
  • DNA-Binding Proteins
  • Phosphoproteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • TATA-Binding Protein Associated Factors
  • TATA-Box Binding Protein
  • Transcription Factor TFIIA
  • Transcription Factors
  • down-regulator of transcription 1
  • Adenosine Triphosphatases
  • MOT1 protein, S cerevisiae
  • DNA Helicases