Abstract
A new extracellular antifungal protein with a yield of 10 mg per liter was isolated from the culture medium of the mould Trichoderma viride. The protein, which we named viridin, was purified by carboxymethyl-cellulose cation-exchange chromatography and Superose 12 HR 10/30 high-performance liquid chromatography. Viridin, a basic protein of approximately 65 kDa as determined by SDS-PAGE, inhibits the growth of the cotton pathogen Verticillum dahliae, the IC50 being 6 microM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Androstenes / chemistry
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Androstenes / isolation & purification*
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Androstenes / pharmacology
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Antifungal Agents / chemistry
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Antifungal Agents / isolation & purification*
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Antifungal Agents / pharmacology
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Bacteriocins / chemistry
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Bacteriocins / isolation & purification*
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Bacteriocins / pharmacology
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Chromatography, Gel
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Chromatography, Ion Exchange
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Gossypium / microbiology
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Molecular Weight
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Trichoderma / chemistry*
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Verticillium / drug effects*
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Verticillium / growth & development
Substances
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Androstenes
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Antifungal Agents
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Bacteriocins
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viridin