Specific Alkylation of Guanine Opposite to a Single Nucleotide Bulge: A Chemical Probe for the Bulged Structure of DNA

K Nakatani; A Okamoto; I Saito I

Specific Alkylation of Guanine Opposite to a Single Nucleotide Bulge: A Chemical Probe for the Bulged Structure of DNA

Angew Chem Int Ed Engl. 1999 Nov 15;38(22):3378-3381.

Authors

K Nakatani¹, A Okamoto, I Saito I

Affiliation

¹ Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University (and) CREST, Japan Science and Technology Corporation, Kyoto 606-8501 (Japan).

PMID: 10602201

Abstract

Remarkable enhancement of guanine alkylation just opposite a bulged site by 13R-1 compared with those in normal duplex DNA is ascribable to the formation of a unique intercalated complex that is attainable only at the bulged sites (see picture). This observation suggests that 13R-1 can be used as a chemical probe for the bulged structure of DNA.