Abstract
A panel of lipid, carbohydrate and protein antibodies were optimized for use in detecting M. leprae antigens in paraffin embedded material. Skin and nerve biopsies from 13 patients across the leprosy spectrum were studied. All antibodies detected antigen in tissues with a BI > 1. Phenolic-glycolipid was not detected in bacteriologically negative tissue but lipoarabinomanan (LAM) and protein antigens were detected. Staining with LAM was strongest and gave least background. The transfer of this immunohistochemical technique to paraffin embedded material will allow examination of tissue with better morphology and from clinics without access to tissue freezing facilities.
Publication types
-
Clinical Trial
-
Controlled Clinical Trial
MeSH terms
-
Antibodies, Monoclonal / analysis
-
Antigens, Bacterial / analysis*
-
Bacterial Proteins*
-
Biomarkers / analysis
-
Biopsy, Needle
-
Chaperonin 60
-
Chaperonins / analysis
-
Culture Techniques
-
Female
-
Glycolipids / analysis
-
Humans
-
Immunohistochemistry
-
Leprosy / immunology
-
Leprosy / pathology*
-
Lipopolysaccharides / analysis
-
Macrophages / chemistry
-
Male
-
Peripheral Nerves / chemistry*
-
Sensitivity and Specificity
-
Skin / chemistry*
-
Skin / immunology
Substances
-
Antibodies, Monoclonal
-
Antigens, Bacterial
-
Bacterial Proteins
-
Biomarkers
-
Chaperonin 60
-
Glycolipids
-
Lipopolysaccharides
-
heat-shock protein 65, Mycobacterium
-
lipoarabinomannan
-
phenolic glycolipid I, Mycobacterium leprae
-
antigen 85B, Mycobacterium leprae
-
Chaperonins