Molecular identification of encephalitozoon hellem in an ostrich

Avian Dis. 1999 Oct-Dec;43(4):779-82.

Abstract

Microsporidia are obligate, intracellular, eukaryotic parasites found in a wide variety of vertebrate and invertebrate hosts. Scientific literature contains a small number of reports of these parasites in psittacine hosts, and recently microsporidiosis was reported in the first nonpsittacine host, an ostrich. DNA was extracted from formalin-fixed, paraffin-embedded ostrich tissues, and a portion of the small subunit ribosomal RNA gene was sequenced to identify the microsporidian species. The organisms were identified as Encephalitozoon hellem, a parasite species that was first described in immunocompromised humans and recently reported in three psittacine species.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Bird Diseases / parasitology*
  • Bird Diseases / pathology
  • DNA, Protozoan / genetics
  • DNA, Protozoan / isolation & purification
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal / isolation & purification
  • Encephalitozoon / classification*
  • Encephalitozoon / genetics*
  • Encephalitozoon / isolation & purification
  • Encephalitozoonosis / pathology
  • Encephalitozoonosis / veterinary*
  • Humans
  • Molecular Sequence Data
  • Parrots
  • RNA, Protozoan / genetics
  • RNA, Ribosomal / genetics
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Struthioniformes / parasitology*

Substances

  • DNA, Protozoan
  • DNA, Ribosomal
  • RNA, Protozoan
  • RNA, Ribosomal