Shiga-toxin producing Escherichia coli from healthy cattle in a semi-urban community in Calcutta, India

Indian J Med Res. 1999 Sep:110:83-5.

Abstract

From October to December 1998, single faecal samples from 67 healthy cattle in a semi-urban community near Calcutta were examined for Shiga toxin producing Esch. coli (STEC) using a multiplex PCR primary screen followed by plating on sorbitol MacConkey agar. STEC was isolated from the faeces of 7 (10.5%) animals. The eight strains isolated belonged to eight serotypes viz, O146:H1, O149:HNT, ONT:H34, ONT:H19, O88:HN, ONT:H2, O82:H8 and O28ac:H21. Bead enzyme linked immunosorbent assay showed that three strains produced Shiga toxin 1, one produced Shiga toxin 2 and four produced both.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins / biosynthesis*
  • Cattle / microbiology*
  • Escherichia coli / metabolism*
  • India
  • Shiga Toxins

Substances

  • Bacterial Toxins
  • Shiga Toxins