Follow-up analysis of translocation and dicentric frequencies, measured by FISH-chromosome painting in breast cancer patients after partial-body radiotherapy with little bone marrow exposure

Mutat Res. 1999 Oct 29;446(1):103-9. doi: 10.1016/s1383-5718(99)00153-9.

Abstract

Follow-up translocation and dicentric measurements in blood lymphocytes of five breast cancer patients were performed by FISH using painting probes for chromosomes 1, 4, and 12 simultaneously with a pancentromeric DNA probe, during 14 months after fractionated photon therapy affecting only small areas of the bone marrow (about 5%). The analysis of individual time-courses for translocations and dicentrics revealed a significant temporal decline of the yields with comparable half-times, both for these stable and unstable aberration types in two patients. In three patients, the aberration yields remained fairly unchanged during the observation period. Regarding retrospective biodosimetry for cases with partial-body exposures or large dose inhomogeneity, it follows that even FISH chromosome painting is limited in assessing initial doses correctly in terms of stable translocations.

MeSH terms

  • Bone Marrow Cells / radiation effects*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / radiotherapy*
  • Breast Neoplasms / surgery
  • Chromosome Aberrations*
  • Chromosome Painting / methods
  • Combined Modality Therapy
  • Dose Fractionation, Radiation
  • Female
  • Follow-Up Studies
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lymphocytes / cytology
  • Lymphocytes / radiation effects
  • Middle Aged
  • Tomography, X-Ray Computed
  • Translocation, Genetic*