Abstract
Two isolates of Streptococcus pneumoniae having different optochin susceptibilities were recovered from a blood sample of a 2-year-old boy with community-acquired pneumonia. The two isolates were documented to belong to a single clone on the basis of the isolates' identical serotype (23F), antibiograms by the E-test, random amplified polymorphic DNA patterns generated by arbitrarily primed PCR, pulsed-field gel electrophoresis, and restriction fragment length polymorphism of the penicillin-binding protein genes pbp2b and pbp2x.
MeSH terms
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Bacteremia / drug therapy
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Bacteremia / microbiology*
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Bacterial Typing Techniques
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Cefazolin / therapeutic use
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Cephalexin / therapeutic use
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Child, Preschool
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Clone Cells
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Community-Acquired Infections
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Electrophoresis, Gel, Pulsed-Field
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Humans
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Male
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Microbial Sensitivity Tests
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Pneumonia, Pneumococcal / drug therapy
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Pneumonia, Pneumococcal / microbiology*
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Quinine / analogs & derivatives*
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Quinine / pharmacology
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Random Amplified Polymorphic DNA Technique
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Serotyping
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Streptococcus pneumoniae / classification
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Streptococcus pneumoniae / drug effects*
Substances
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ethylhydrocupreine
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Quinine
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Cefazolin
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Cephalexin