Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP-dependent protein kinase II(1)

FEBS Lett. 1999 Dec 31;464(3):174-8. doi: 10.1016/s0014-5793(99)01585-9.

Abstract

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIbeta and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism*
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / metabolism*
  • Ligands
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Rats
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Substrate Specificity

Substances

  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing
  • Akap9 protein, rat
  • Carrier Proteins
  • Cytoskeletal Proteins
  • Ligands
  • Receptors, N-Methyl-D-Aspartate
  • Recombinant Proteins
  • Cyclic AMP-Dependent Protein Kinases

Associated data

  • GENBANK/AF133906