Abstract
While studying the neural precursor cell intermediate filament protein known as nestin in the developing mouse brain, we observed a strong cross-reaction of our nestin antibody with a 50 kDa protein that appeared on embryonic day 10 and continued to accumulate until postnatal day 1. Here we report evidence that this protein is a brain-specific variant form of alpha-tubulin and discuss its implications.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Brain / metabolism*
-
Cross Reactions
-
Epitopes, B-Lymphocyte / immunology
-
Female
-
Genetic Variation
-
Intermediate Filament Proteins / immunology
-
Male
-
Mice
-
Mice, Inbred ICR
-
Molecular Sequence Data
-
Nerve Tissue Proteins*
-
Nestin
-
Neurons / metabolism
-
Olfactory Bulb / metabolism
-
Rabbits
-
Rats
-
Rats, Sprague-Dawley
-
Species Specificity
-
Stem Cells / metabolism
-
Tubulin / immunology
-
Tubulin / metabolism*
-
Tumor Cells, Cultured
Substances
-
Epitopes, B-Lymphocyte
-
Intermediate Filament Proteins
-
Nerve Tissue Proteins
-
Nes protein, mouse
-
Nes protein, rat
-
Nestin
-
Tubulin