Gene walking by PCR amplification of short fragments from Taq DNA polymerase--modified P1 plasmid DNA and TA cloning

Biotechniques. 1999 Dec;27(6):1118-20, 1122. doi: 10.2144/99276bm06.
No abstract available

MeSH terms

  • Animals
  • Bacteriophage P1
  • Cloning, Molecular / methods
  • DNA / genetics
  • DNA / metabolism*
  • DNA Primers
  • Electrophoresis, Agar Gel
  • Gene Amplification*
  • Glycoproteins / genetics
  • Membrane Proteins
  • Plasmids / genetics
  • Polymerase Chain Reaction / methods*
  • Rats
  • Reproducibility of Results
  • Taq Polymerase / metabolism*

Substances

  • DNA Primers
  • Glycoproteins
  • Membrane Proteins
  • Pvr protein, rat
  • DNA
  • Taq Polymerase