Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoprotein

J Rheumatol. 2000 Jan;27(1):128-34.

Abstract

Objective: To examine a well characterized group of patients with systemic lupus erythematosus (SLE) for anti-calreticulin.

Methods: The sera of 77 patients with SLE were studied by immunodiffusion, solid phase immunoassay, and immunoblot for antibodies against ribonucleoprotein (RNP) autoantigens and calreticulin.

Results: Thirty-five had anti-calreticulin and 40 had anti-60 kDa Ro. There was no association of anti-60 kDa Ro and anti-calreticulin. However, among anti-60 kDa Ro positive sera that also contained either anti-La or anti-RNP, none of 18 had anti-calreticulin. All the remaining sera with anti-60 kDa Ro had anti-calreticulin and anti-52 kDa Ro.

Conclusion: Anti-60 kDa Ro patients with SLE can be divided into those with anti-60 kDa Ro and either anti-La or anti-RNP or those with anti-60 kDa Ro, anti-52 kDa Ro, and anti-calreticulin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoantigens / blood*
  • Autoantigens / immunology*
  • Calcium-Binding Proteins / immunology*
  • Calreticulin
  • Humans
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology*
  • RNA, Small Cytoplasmic*
  • Ribonucleoproteins / immunology*
  • SS-B Antigen
  • Transcription Factors / immunology*

Substances

  • Autoantigens
  • Calcium-Binding Proteins
  • Calreticulin
  • RNA, Small Cytoplasmic
  • RO60 protein, human
  • Ribonucleoproteins
  • SS-A antigen
  • Transcription Factors