High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid

F Barloy-Hubler; D Capela; M J Barnett; S Kalman; N A Federspiel; S R Long; F Galibert

doi:10.1128/JB.182.4.1185-1189.2000

High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid

J Bacteriol. 2000 Feb;182(4):1185-9. doi: 10.1128/JB.182.4.1185-1189.2000.

Authors

F Barloy-Hubler¹, D Capela, M J Barnett, S Kalman, N A Federspiel, S R Long, F Galibert

Affiliation

¹ Laboratoire de Recombinaisons Génétiques UPR41-CNRS, Faculté de Médecine, F-35043 Rennes Cedex, France.

Abstract

To facilitate sequencing of the Sinorhizobium meliloti 1021 pSyma megaplasmid, a high-resolution map was constructed by ordering 113 overlapping bacterial artificial chromosome clones with 192 markers. The 157 anonymous sequence tagged site markers (81,072 bases) reveal hypothetical functions encoded by the replicon.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chromosomes, Bacterial / genetics
Physical Chromosome Mapping*
Plasmids / genetics*
Sequence Tagged Sites
Sinorhizobium meliloti / genetics*