Comparison of blood collected in acid-citrate-dextrose and EDTA for use in human immunodeficiency virus peripheral blood mononuclear cell cultures

J Clin Microbiol. 2000 Feb;38(2):858-60. doi: 10.1128/JCM.38.2.858-860.2000.

Abstract

Paired blood samples collected in acid-citrate-dextrose and EDTA were compared for human immunodeficiency virus (HIV) infectivity on the day of collection or after 1 day of storage at room temperature. No significant differences between the anticoagulants were observed. Culture positivity was significantly associated with HIV RNA viral loads for both anticoagulants.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anticoagulants*
  • Blood Coagulation
  • Blood Specimen Collection
  • Cells, Cultured
  • Citric Acid*
  • Edetic Acid*
  • Glucose / analogs & derivatives*
  • HIV-1 / physiology*
  • Humans
  • Leukocytes, Mononuclear / virology*
  • RNA, Viral / blood
  • Viral Load

Substances

  • Anticoagulants
  • RNA, Viral
  • acid citrate dextrose
  • Citric Acid
  • Edetic Acid
  • Glucose