Cloning and expression in Escherichia coli of 2-hydroxypropylphosphonic acid epoxidase from the fosfomycin-producing organism, Pseudomonas syringae PB-5123

Biosci Biotechnol Biochem. 1999 Dec;63(12):2222-4. doi: 10.1271/bbb.63.2222.

Abstract

The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase from fosfomycin-producing Streptomyces wedmorensis. The purified recombinant protein of this ORF converted 2-hydroxypropylphosphonic acid to fosfomycin. This result clearly showed the ORF to encode 2-hydroxypropylphosphonic acid epoxidase in PB-5123.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Cloning, Molecular
  • Escherichia coli
  • Fosfomycin / biosynthesis*
  • Molecular Sequence Data
  • Open Reading Frames
  • Oxidoreductases / biosynthesis*
  • Oxidoreductases / genetics*
  • Pseudomonas / enzymology*

Substances

  • Bacterial Proteins
  • FosC protein, Pseudomonas syringae
  • Fosfomycin
  • Oxidoreductases
  • 2-hydroxypropylphosphonic acid epoxidase

Associated data

  • GENBANK/D82818