Abstract
To determine the frequency of in vivo activated T(H)1 lymphocytes, T-cell subsets of 9 multiple sclerosis patients with active disease and 17 healthy controls were analyzed by immunostaining for CCR5, CD26, and their expression of interleukin-2, interferon-gamma, and tumor necrosis factor-alpha. The numbers of CCR5+ interferon-gamma- and tumor necrosis factor-alpha-producing T cells were significantly increased in the peripheral blood of multiple sclerosis patients. CCR5 expression may be a useful marker to identify effector cells in multiple sclerosis and could be used as a tool for monitoring disease activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Dipeptidyl Peptidase 4 / metabolism
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Female
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Humans
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Interferon-gamma / metabolism*
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Ionomycin / pharmacology
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Lymphocyte Count
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Male
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Middle Aged
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Multiple Sclerosis / metabolism*
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Multiple Sclerosis / pathology
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Receptors, CCR5 / metabolism*
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Reference Values
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T-Lymphocytes / drug effects
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T-Lymphocytes / metabolism*
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T-Lymphocytes / pathology*
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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Receptors, CCR5
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Tumor Necrosis Factor-alpha
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Ionomycin
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Interferon-gamma
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Dipeptidyl Peptidase 4
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Tetradecanoylphorbol Acetate