Production of chimeric antibody in cells and larvae of silkworm

Chin J Biotechnol. 1999;15(1):37-42.

Abstract

By using the recombinant baculovirus expression system, the chimeric antibody against human small cell lung carcinoma was expressed in the cells and larvae of silkworm Bomyx mori N (BmN). The chimeric antibodies produced in BmN cells and larvae which were infected with recombinant viruses rNPVL2, rNPVH17 and dual-recombinant virus rNPVLH19 were detected by Western blot analysis. The co-expression products of heavy- and light-chain genes in silkworm larvae revealed stronger immune binding activity than the single product of heavy or light chain gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / metabolism*
  • Blotting, Western
  • Bombyx / genetics
  • Bombyx / metabolism*
  • Bombyx / virology
  • Carcinoma, Small Cell / immunology*
  • Genetic Vectors
  • Humans
  • Larva
  • Lung Neoplasms / immunology*
  • Nucleopolyhedroviruses / genetics
  • Protein Engineering
  • Recombinant Fusion Proteins / metabolism*

Substances

  • Antibodies
  • Recombinant Fusion Proteins