Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts

J Hoevers; P Holman; K Logan; M Hommel; R Ashford; K Snowden

doi:10.1007/s004360050010

Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts

Parasitol Res. 2000 Jan;86(1):57-61. doi: 10.1007/s004360050010.

Authors

J Hoevers¹, P Holman, K Logan, M Hommel, R Ashford, K Snowden

Affiliation

¹ College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.

PMID: 10669137
DOI: 10.1007/s004360050010

Abstract

Genomic diversity among 14 isolates of Blastocystis hominis from 4 different geographic locations was examined by small-subunit rRNA (ssu rRNA) restriction-fragment-length polymorphisms (RFLP) using 5 different restriction endonucleases. On the basis of the observed RFLP patterns among the isolates, a total of 12 genotypes were identified, with 7 isolates exhibiting mixed RFLP genotypes. There was no correlation between B. hominis geographic origin and RFLP banding pattern or genotype.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocystis / classification*
Blastocystis / genetics*
Blastocystis / isolation & purification
Blastocystis Infections / parasitology
Deoxyribonucleases, Type II Site-Specific
Genes, Protozoan
Genes, rRNA / genetics*
Humans
Polymorphism, Restriction Fragment Length*
RNA, Ribosomal / genetics

Substances

RNA, Ribosomal
Deoxyribonucleases, Type II Site-Specific