Use of linear and multiple antigenic peptides in the immunodiagnosis of acute hepatitis A virus infection

J Immunol Methods. 2000 Feb 3;234(1-2):23-34. doi: 10.1016/s0022-1759(99)00196-9.

Abstract

The reactivities of two panels of anti-HAV human sera from geographically distinct areas (Chile and Spain) to synthetic peptides from the VP1, VP2 and VP3 hepatitis A virus capsid proteins were examined by an enzyme-linked immunosorbent assay (ELISA) procedure. Two and four branched multiple antigenic peptides (MAPs) and palmitoylated peptides were compared with free synthetic sequences for the detection of IgM anti-HAV antibodies in the two panels of human sera. Our results showed that acute hepatitis A patient sera recognized preferentially homogeneous two branched MAPs and palmitic acid conjugated peptides. The palmitoyl-derived VP3(110-121) peptide and the corresponding dimeric MAP were the most sensitive and appropriate for serological studies of HAV-infected patients by ELISA, sensitivity and specificity being higher than 90% and 95%, respectively. These peptide-based tests open up new avenues in the development of peptide-based immunosorbent assays for the detection of acute HAV disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antigens, Viral / immunology*
  • Capsid / chemical synthesis
  • Capsid / immunology*
  • Capsid Proteins
  • Enzyme-Linked Immunosorbent Assay / methods
  • Epitopes, B-Lymphocyte / immunology
  • Hepatitis A / blood
  • Hepatitis A / diagnosis*
  • Hepatitis A / immunology
  • Hepatitis A Antibodies
  • Hepatitis A Antigens
  • Hepatitis Antibodies / blood
  • Hepatitis Antibodies / immunology
  • Humans
  • Immunologic Tests / methods
  • Palmitic Acid / immunology
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology*
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Reagent Kits, Diagnostic
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Structural Proteins / chemical synthesis
  • Viral Structural Proteins / immunology*

Substances

  • Antigens, Viral
  • Capsid Proteins
  • Epitopes, B-Lymphocyte
  • Hepatitis A Antibodies
  • Hepatitis A Antigens
  • Hepatitis Antibodies
  • Peptide Fragments
  • Peptides
  • Reagent Kits, Diagnostic
  • VP1 protein, hepatitis A virus
  • VP3 peptide (110-121), hepatitis A virus
  • Viral Structural Proteins
  • Palmitic Acid